肌肉来源干细胞表面抗原-1阳性与阴性细胞体外培养成肌特性的比较
A comparison of the myogenic characteristics between stem cell antigen-1 + and stem cell antigen-1- cells derived from skeletal muscle
作者：车晓霞 赵彤 朱玲玲 郭杰
Author：Che Xiaoxia1, Zhao Tong2, Zhu Lingling2, Guo Jie3
收稿日期：2011-08-25 年卷（期）页码：2011,29(04):369-369-371
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：干细胞表面抗原-1,肌肉干细胞,成肌调节因子,细胞周期,
Key words：stem cell antigen-1,muscle stem cell,muscle regulatory factor,cell cycle,
基金项目：
北京市自然科学基金资助项目（7072029）；山东省科技攻关基金资助项目（2007GG30002035）
中文摘要
目的探讨干细胞表面抗原－1（Sca-1）在成肌细胞增殖分化中的作用。方法采用流式荧光细胞分选技术，从C57BL/6小鼠骨骼肌中分离Sca-1+与Sca-1-细胞进行体外培养，使用CCK-8试剂盒检测2种细胞的增殖曲线，Westernblot测定2种细胞在体外培养过程中Sca-1、MyoD、成肌素（Myogenin）的蛋白表达。结果在体外培养的前3 d，Sca-1+与Sca-1-细胞增殖曲线没有明显差别；3 d后，Sca-1-细胞增殖比Sca-1+细胞明显加速。同时，2种细胞在体外培养过程中Sca-1均没有明显表达，而Sca-1+细胞的成肌调节因子表达比Sca-1－细胞显著增加。结论Sca-1的表达在细胞生长发育过程中具有时段性，与成肌细胞细胞周期的起止有关。
英文摘要
Objective To probe into the function of stem cell antigen-1（Sca-1） in cell proliferation and differentiation. Methods Sca-1+ and Sca-1- muscle derived cells were separated from C57BL/6 mice by fluorescence-activated cell sorting and then cultured in vitro. After 5 days cells proliferative curve were drawn according CCK-8 experimental results. Western blot also were done to detect Sca-1, MyoD and Myogenin expression in cultured Sca-1+ and Sca-1- muscle derived cells. Results The difference of the proliferative curve of Sca-1+ and Sca-1- muscle derived cells cultured 3 days in vitro was not apparent, but Sca-1- muscle derived cells had a accelerated division rate in the follow days compared to the Sca-1+ muscle derived cells. Sca-1 expression in both cells was not obvious. MyoD and Myogenin expression were stronger in Sca-1+ than Sca-1- muscle derived cells. Conclusion Sca-1 expression in muscle derived cells takes a period of time that related to the beginning and ending of the cell cycle.
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