抗人血管内皮生长因子单克隆抗体抑癌作用的形态学观察及作用机制探讨
Morphological Observation of Buccal Mucosa Carcinoma Inhibited by Monoclonal Antibodies of Human Vascular Endothelial Growth Factor
作者：房思炼,王大章,杨西川,何志秀,张杰,郑光勇
Author：Fang Silian, Wang Dazhang, Yang Xichuan,et al
收稿日期：2002-04-25 年卷（期）页码：2002,20(02):138-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：血管内皮生长因子,单克隆抗体,肿瘤,血管生成,形态学,
Key words：VEGF,angiogenesis,tumor,monoclonalantibody,morphology,
基金项目：本课题为国家自然科学基金资助项目(编号39970798)
中文摘要
目的:研究抗人血管内皮生长因子(VEGF)单抗E11抑制颊癌组织的形态学改变,并探讨E11抑癌效应的机制。方法:接种人颊鳞癌的BALBPc裸小鼠分别通过腹腔或瘤周皮下注射抗人VEGF单抗或生理盐水。接种后第18天处死,取颊癌组织进行光镜及透射电镜观察。结果:E11组颊癌组织中血管分布稀少、存在囊性变坏死区及癌细胞坏死、凋亡等。此外还有血管内皮细胞变性、凋亡及血管壁受破坏、管腔变窄和堵塞等血管系统的异常改变。而生理盐水组癌组织血管丰富,颊癌组织结构完整。结论:抗人VEGF单抗是通过抑制颊癌的血管生成而产生抑癌效应。
英文摘要
Objective:The purpose of this studywas to investigate the mechanism of the inhibited effects of anti-human vascularendothe- lial growth factor (VEGF) monoclonal antibodies E11on the growth of buccal mucosal carcinoma.Methods:E11was hypodermic and celiac injected into BALBPc nuPnumice, whichwere transplantedwith buccal carcinoma. The saline was injected as the nega- tive control. Mice were killed on the 18th day and the pathological alteration of tumor was evaluated.Results:Microscopically, massive necrosis and decreased vascular density was observed in tumor tissues treated with E11. Correspondentwith the aforemen- tioned phenomena, degeneration and apoptosis was also found in the tumor cells. For the first time, degeneration and destruction of the vascular endothelial cells in the tumor tissues treated with E11were observed using microscopy and transmission electron mi- croscopy examination.Conclusion:The study demonstrated that E11inhibited the tumor angiogenesis by blocking the action of VEGF and resulted in significantinhibitory effects on the tumorgrowth. The resultwould offer a therapeutic base to the clinical ap- plication of the anti-human VEGF monoclonal antibodies.
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