期刊导航

论文摘要

不同硫酸化程度的糖胺聚糖成软骨作用的体外研究

Effect of glycosaminoglycans with different degrees of sulfation on chondrogenesis

作者:郑雯, 蔡明详, 彭荟桢, 刘敏义, 刘湘宁

Author:Zheng Wen, Cai Ming-xiang, Peng Huizhen, Liu Minyi, Liu Xiangning

收稿日期:2023-02-17          年卷(期)页码:2023,41(4):395-395-404

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:软骨形成,软骨损伤修复,硫酸化糖胺聚糖,硫酸化程度,

Key words:chondrogenesis,cartilage repair,sulfated glycosaminoglycan,degree of sulfation,

基金项目:国家自然科学基金资助项目(82201001);广东省自然基金项目(2021A1515010882);广州市科技计划项目(2021020-10040);暨南大学附属第一医院临床前沿新技术立项项目(a01210)

中文摘要

目的 本研究旨在探讨硫酸软骨素(CS)、硫酸皮肤素(DS)与肝素(HEP)对软骨形成细胞成软骨分化和小鼠关节软骨状态维持的作用及其机制。 方法 小鼠软骨发生细胞系(ATDC5)和小鼠关节软骨组织块在含不同硫酸化程度糖胺聚糖的培养基中培养后,使用细胞增殖试验、阿利新蓝染色、实时荧光定量聚合酶链反应(RT-qPCR)和蛋白质印迹(Western blot)分析来观察细胞增殖、成软骨分化、软骨形成、软骨组织维持的作用,并进一步探讨其潜在机制。 结果 HEP和DS主要通过激活骨形态发生蛋白(BMP)信号通路,CS主要通过激活蛋白激酶B(AKT)信号通路促进软骨形成细胞的增殖能力、提高基质蛋白多糖的生成、增加Sox9、Ⅱ型胶原蛋白(Col2a1)和聚集蛋白聚糖(Aggrecan)的表达水平。 结论 本研究探讨了不同硫酸化程度的糖胺聚糖对细胞成软骨与软骨稳态的维持作用差异及其机制,HEP有助于促进软骨形成和维持软骨组织正常状态,CS在损伤软骨组织再生方面效果更好。

英文摘要

ObjectiveThis study aims to investigate the effects and mechanisms of chondroitin sulfate (CS), dermatan sulfate (DS), and heparin (HEP) on chondrogenesis of murine chondrogenic cell line (ATDC5) cells and the maintenance of murine articular cartilagein vitro.MethodsATDC5 and articular cartilage tissue explant were cultured in the medium containing different sulfated glycosaminoglycans. Cell proliferation, differentiation, cartilage formation, and mechanism were observed using cell proliferation assay, Alcian blue staining, real-time quantitative polymerase chain reaction (RT-qPCR), and Western blot, respectively.ResultsResults showed that HEP and DS primarily activated the bone morphogenetic protein (BMP) signal pathway, while CS primarily activated the protein kinase B (AKT) signal pathway, further promoted ATDC5 cell proliferation and matrix production, and increased Sox9, Col2a1, and Aggrecan expression.ConclusionThis study investigated the differences and mechanisms of different sulfated glycosaminoglycans in chondrogenesis and cartilage homeostasis maintenance. HEP promotes cartilage formation and maintains the normal state of cartilage tissuein vitro, while CS plays a more effective role in the regeneration of damaged cartilage tissue.

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