重组人骨形成蛋白2对人牙周膜成纤维细胞的生物学作用
The Biological Effects of Recombinant Human Bone Morphogenetic Protein 2 on Human Periodontal Ligament Fibroblasts
作者：司晓辉 刘正
Author：Si Xiaohui,Liu Zheng
收稿日期：2002-02-25 年卷（期）页码：2002,20(01):10-10-12.
摘要
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：重组人骨形成蛋白2,人牙周膜成纤维细胞,碱性磷酸酶,骨钙素,矿化,
Key words：recombinant human bone morphogenetic protein 2 ,human periodontal ligament fibroblasts ,alkaline
phosphatase,osteocalcin ,mineralization,
基金项目：
本课题为国家自然科学基金资助项目(编号30000191)
中文摘要
目的:研究重组人骨形成蛋白2 (rhBMP2) 对人牙周膜成纤维细胞(HPDLFs) 的生物学作用。方法:原代培养HPDLFs ,并观察rhBMP2 对HPDLFs 增殖、碱性磷酸酶(ALP) 活性、骨钙素(OC) 合成及矿化结节形成的作用。结果:0125～2 mgPml rhBMP2 对HPDLFs 的增殖无明显作用,015～2 mgPml rhBMP2 显著提高HPDLFs 的ALP 活性,刺激OC合成和矿化结节形成。结论:rhBMP2 对HPDLFs 的作用具有剂量依赖性。rhBMP2 能够刺激HPDLFs 表达成骨细胞表型并促进其表型成熟。
英文摘要
The purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs) . Methods : Human periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation , alkaline phosphatase (ALP) activity , osteocalcin (OC) synthesis and formation of the mineralized nodules. Results : rhBMP2 (0125～2 mgPml) had no remarkable effect on the prolifera2 tion of HPDLFs. The ALP activity , OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 015～2 mgPml rhBMP2. Conclusion : The effects of rhBMP2 on HPDLFs are dose- dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs.
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