ObjectiveTo explore the dynamic changes of human papillomavirus (HPV) type 16E5 gene in the development of cervical cancer and the significance ofE5 mRNA in early screening of cervical cancer.MethodsParaffin specimens of cervical lesions were collected from 49 cases (HPV positive) during September 2015 to December 2017 According to the standard of FIGO, all cervical lesions were diagnosed as: 13 cases of cervicitis, cervical intraepithelial neoplasia disorders (CIN) Ⅰ in 5 cases, CIN Ⅱ in 18 cases, CIN Ⅲ in 5 cases, 8 cases of cervical cancer. Real-time fluorescence quantitative PCR was used to detect the integrity ofE5 gene and the mRNA expression levels ofE5,E6 andE7in cervical tissues.ResultsAll the 49 cases showed positive HPV16 infection.E5 genetic integrity in CINⅠwas higher than that in cervical inflammation, CIN Ⅱand cervical cancer (P< 0.05), which was also higher than that in cin ⅲ, but without statistically significance (P>0.05). The mRNA levels ofE5,E6,E7 were the highest in CIN Ⅲ. Compared withE6 andE7,E5 presented superior expression in all types of cervical lesions (P< 0.05), whileE6andE7 mRNA expressions only increased in CIN Ⅲ and cervical cancer.ConclusionIn the patients with HPV16 infection, the integrity ofE5 gene in cervical tissues may be related to the occurrence and development of cervical diseases.E5 gene is expected to be the target gene for early screening of cervical cancer.
