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论文摘要

PGC-1ɑ在多囊卵巢综合征模型大鼠卵巢组织的表达变化

Expression of PGC-1ɑ in Ovarian Tissue of PCOS Rat Model

作者:郭琴, 高瑞萍, 陶莹

Author:GUO Qin, GAO Rui-ping, TAO Ying

收稿日期:2019-10-08          年卷(期)页码:2020,51(6):817-821

期刊名称:四川大学学报(医学版)

Journal Name:JOURNAL OF SICHUAN UNIVERSITY (MEDICAL SCIENCE EDITION)

关键字:PGC-1ɑ, 肥胖PCOS大鼠模型, 颗粒细胞, 高脂饮食

Key words:PGC- 1ɑ, Obesity PCOS rat model, Granulosa cells, High fat-diet

基金项目:

中文摘要

目的 探讨多囊卵巢综合征(polycystic ovary syndrome, PCOS)大鼠模型及肥胖PCOS大鼠模型卵巢组织过氧化物酶体增殖物激活受体γ辅助活化因子1α(PGC-1α)的表达,以及PCOS 发生的可能机制。 方法 SD大鼠30只,随机分为对照组、PCOS组及肥胖PCOS组,PCOS两组大鼠每日一次予脱氢表雄酮〔DHEA,60 mg/(kg·d)〕溶于0.2 mL注射用大豆油中皮下注射,共21 d,制备PCOS模型,肥胖PCOS模型组大鼠在DHEA制模基础上加入高脂饮食,每组大鼠各10只。造模前(0 d)和造模后第22天称体质量,取腹主动脉血检测血清睾酮(testosterone,T)水平,取大鼠卵巢组织,HE染色观察组织形态学改变,免疫组织化学染色和Western blot检测PGC-1ɑ蛋白表达。 结果 造模前3组大鼠体质量差异无统计学意义,造模后第22天,大鼠增加的体质量以及大鼠血清 T 质量浓度,均为肥胖PCOS组>PCOS组>对照组,差异有统计学意义(P<0.05或P<0.01)。大鼠卵巢组织HE染色结果显示,对照组大鼠卵巢组织镜下可见不同发育时期的卵泡和少量黄体,颗粒细胞排列多为4~6层;PCOS组及肥胖PCOS组大鼠卵巢组织中未成熟的小卵泡数量明显增加,颗粒细胞1~3层排列、较松散,部分卵泡闭锁,肥胖PCOS组大鼠卵巢闭锁卵泡直径增大,颗粒细胞层数减少,卵母细胞消失等现象更明显。免疫组化染色结果显示,对照组大鼠卵巢组织PGC-1ɑ蛋白阳性表达主要分布于卵丘及颗粒细胞。从PGC-1ɑ蛋白在卵巢组织的表达平均灰度均值来看,PCOS组(0.53±0.06)和肥胖PCOS组(0.36±0.03)均低于对照组(0.75±0.03),差异有统计学意义(P<0.05),肥胖PCOS组PGC-1ɑ表达低于PCOS组(P<0.01)。Western blot检测结果与免疫组化染色结果一致。 结论 PGC-1ɑ表达降低与高脂环境下卵巢颗粒细胞损伤有关。PGC-1ɑ在卵巢卵泡的颗粒细胞中表达降低可能是PCOS发生发展的重要原因。

英文摘要

ObjectiveTo investigate the expression of peroxisome proliferator-activated receptor γ co-activator 1α (PGC-1α) in the ovarian tissue of polycystic ovary syndrome (PCOS) rat model and obese PCOS rat model, and the possible mechanism of PCOS.MethodsThirty SD rats were randomly divided into control group, PCOS rat model group and obese PCOS rat model group. DHEA dissolved in 0.2 mL soybean oil was injected daily into the rats of two PCOS groups for 21 d. Rats in obese PCOS model group were added with high-fat diet based on DHEA modeling, and each group had 10 rats. Body mass were measured before and on the 22ndday after modeling. The serum testosterone (T) levels were measured by abdominal aortic blood, and the ovarian tissues of rats were taken for histological changes were observed by HE staining, immunohistochemical staining and Western blot to detect PGC-1ɑ protein expression.ResultsThe body mass of rats in each group increased after modeling, and the body mass of rats in PCOS group and obese PCOS group increased significantly (P<0 .05). the serum t concentration of two pcos model groups was higher than that of control group (P<0 .01). the serum t concentration in obese pcos model group was higher than that in the pcos group (P<0 .05). the results of he staining of rat ovarian tissue showed that there were follicles and a small amount of corpus luteum at different developmental stages in the control group, and the granulosa cells were arranged in 4-6 layers. the number of immature small follicles in the two pcos groups was significantly increased. the granulosa cells were arranged in 1-3 layers, relatively looser, and some follicles were atresia. in the obese pcos group, the diameter of ovarian atretic follicles increased, the number of granulocyte layers decreased, and oocytes disappeared more obviously. immunohistochemical staining showed that the pgc-1ɑ protein was mainly expressed in the cumulus and granulosa cells of ovarian tissue in the control group. the mean gray level of pgc-1ɑ protein expression in pcos group (0.53±0.06) and obese pcos group (0.36±0.03) was lower than that of the control group (0.75±0.03), with the statistical difference (P<0 .05). pgc-1ɑ expression in the obese pcos group was lower than that in the pcos group (P<0 .01). the results of western blot were consistent with those of immunohistochemical staining.ConclusionPGC-1ɑ is associated with damage of ovarian granulosa cells in high-fat environment. The decrease of PGC-1ɑ expression in granulosa cells of ovarian follicles may be an important cause of PCOS development.

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