牦牛德尔卑沙门氏菌的分离、鉴定及致病性研究
Isolation, identification and pathogenicity experiment of Salmonella enterica Serovar Derby from yaks (Poephagus grunniens) with diarrhea in Tibetan Qiang Autonomous Prefecture of Ngawa, China
作者：冯兰(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)；安添午(四川省草原科学研究院)；崔鹏飞(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)；赵晓东(四川省草原科学研究院)；李华德(四川省草原科学研究院)；付雪(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)；张兰(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)；翟亚如(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)；李豪(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)；晏文俊(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)；王红宁(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)；杨鑫(四川大学生命科学学院 动物疫病防控与食品安全四川省重点实验室，生物资源与生态环境教育部重点实验室)
Author：Feng Lan(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)；AN Tian-Wu(Sichuan Academy of Grassland Sciences)；CUI Peng-Fei(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)；ZHAO Xiao-Dong(Sichuan Academy of Grassland Sciences)；LI Hua-De(Sichuan Academy of Grassland Sciences)；FU Xue(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)；ZHANG Lan(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)；ZHAI Ya-Ru(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)；LI Hao(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)；YAN Wen-Jun(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)；WANG Hong-Ning(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)；YANG Xin(Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University)
收稿日期：2020-03-04 年卷（期）页码：2020,57(5):1022-1028
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：牦牛； 德尔卑沙门氏菌； 多重耐药； 小鼠致病性实验
Key words：Yaks; Salmonella Derby; Multi-Resistance; Pathogenicity
基金项目：四川省科技厅牦牛产业链技术研究集成与产业化示范项目（2016NZ0005）
中文摘要
为调查四川省阿坝州红原县牦牛腹泻病因，本课题组从红原县4所养殖场采集牦牛腹泻粪便16份，并对分离株进行多位点序列分型、血清型鉴定、耐药性试验及小鼠致病性试验.结果表明：16份样品共计分离出16株沙门氏菌，均为德尔卑沙门氏菌ST71型，100%菌株对四环素、氨苄西林、氯霉素和头孢噻呋多重耐药；选取腹泻较严重牦牛粪样沙门氏菌分离株（编号15XLR）进行致病性试验，得出分离株15XLR的半致死剂量为1.2×107 CFU，病理切片结果显示小鼠后脾脏、肠道损伤严重.本研究对红原县牦牛腹泻病因进行了调查研究，并证实德尔卑沙门氏菌除感染猪鸡外,其能够入侵牦牛机体，提示牦牛腹泻疾病的防控应注重沙门氏菌的感染以及在牦牛群体中的传播.
英文摘要
In order to investigate the pathogen of yaks diarrhea, 16 feces were collected from 4 farms in Hongyuan, Tibetan Qiang Autonomous Prefecture of Ngawa, China. Multilocus sequence typing, serotyping, antimicrobial resistance and pathogenicity experiment were carried out. A total of 16 Salmonella were selected, all strains were Salmonella Derby ST71 and multi-resistant to tetracycline, ampicillin, chloramphenicol and ceftiofur. Salmonella isolated from bloody stool (No. 15XLR) was selected for pathogenicity experiment and the result showed that the half lethal dose of 15XLR was 1.2 × 107 CFU, which can cause severe damage to spleen and intestine according to HE slice. This study investigated the causes of diarrhea in yaks in Hongyuan County, and confirmed that Salmonella Derby can invade yaks except pigs and chickens, suggesting that the prevention and control of diarrhea in yaks should pay attention to infected and the spread of Salmonella in yak population.
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