In order to explore the impact of subalpine highway in Wolong Nature Reserve on alien plants and the potential invasion risk of alien plants along the highway, the plants along the subalpine highway of Wolong Nature Reserve including Gengda to Dengsheng section of G305 were investigated. In the area, 54 alien plants belonging to 51 Genera and 35 families were found. Among them, 5 families including Compositae account for 35.19%.The most of the alien plans was herbaceous, accounting for 62.96%. The plants from Asia except Wolong Nature Reserve accounted for 64.81% and 24.07% were from China except Wolong Nature Reserve. The α diversity index indicated that the farther away from the highway, the fewer alien plants were distributed. Then, an invasive risk assessment system for alien species of Wolong Nature Reserve was established by Analytic Hierarchy Process, and the invasive risk of 54 alien plants were evaluated. It was confirmed that 4 plants had a potential high risk of invasion, 9 plants had a potential medium risk of invasion, and 6 plants had a potential low risk of invasion. Combined with the invasion risk level of alien plants, the corresponding control measures were put forward.