1,4,7-三氮杂环壬烷合钴与牛血清白蛋白的相互作用
On the interaction of 1,4,7-Triazacyclononan -Co(Ⅱ)with Bovine Serum Albumin
作者：祁娟(乐山师范学院化学学院)；王雯雯(乐山师范学院化学学院)；刘凡(乐山师范学院化学学院)
Author：QI Juan(College of Chemistry, Leshan Normal University)；WANG Wen-Wen(College of Chemistry, Leshan Normal University)；LIU Fan(College of Chemstry, Leshan Normal University)
收稿日期：2014-11-17 年卷（期）页码：2016,53(2):397-402
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：1,4,7-三氮杂环壬烷合钴 牛血清白蛋白 荧光光谱 结合位点数 结合常数
Key words：1,4,7-Triazacyclononan -Co(Ⅱ);bovine serum albumin; fluorescence spectroscopy; Binding numbers;binding constants
基金项目：其它
中文摘要
利用荧光光谱法研究了1,4,7-三氮杂环壬烷合钴(Ⅱ)与牛血清白蛋白的相互作用，实验结果表明1,4,7-三氮杂环壬烷合钴(Ⅱ)对牛血清白蛋白的荧光猝灭是静态猝灭，并得出在水溶液和胶束溶液中1,4,7-三氮杂环壬烷合钴(Ⅱ)与牛血清白蛋白作用的结合点位数、结合常数和热力学参数，△H>0，△S>0，其分子间的作用力主要表现为疏水作用力。
英文摘要
The interaction of 1,4,7-Triazacyclononan -Co(Ⅱ) (Cyclen-Co(Ⅱ))with bovine serum albumin (BSA) has been investigated by fluorescence method. The results show that the Cyclen-Co(Ⅱ) strongly quenched fluorescence of BSA. It was proved that the fluorescence quenching mechanism belonged to the static quenching by Stern-Volmer equation. According to Lineweaver-Burk equation and thermodynamics equation, the binding constants,binding sates and thermodynamics parameters were obtained at different temperatures in the aqueous solution and in the solutions of Brij35、CTAB、SDS. The hydrophobic interaction forces played a main role in the binding of Cyclen-Co(Ⅱ) with BSA.
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