In order to investigate the effects of BRI1 gene in Brassica napus (BnBRI1), the over-expression vector pFGC5941-BnBRI1 and the BnBRI1 targeted RNA interference (RNAi) expression vector pFGC5941-BnBRI1-Ri were constructed and transferred into Brassica napus by Agrobacterium tumefaciens. One over-expression transgenic plant and seventeen RNAi transgenic plants were obtained by PCR. RT-PCR analysis showed that, compared with the wild-type “W679”, the expression level of BnBRI1 in over-expression transgenic plant had increased, while the expression level of BnBRI1 in RNAi transgenic plants were down-regulated. RNA interference the expression of BnBRI1 leading to dwarf plants. Dwarf plants will be used for the genetic improvement of rapeseed, and it conducive to mechanized production.
