Objective The present study was designed to determine whether pre-extraction jiggling force could affect the viability and quantity of periodontium on autotransplantation teeth. Methods The subjects consisted of 10 males and 10 females. Jiggling force was applied to the treated teeth before treated premolars were extracted. Control premolars were extracted without any force. The specimens were decalcificated and stained with hematoxylin and eosin and modified Mallory′s trichrome. The area of periodontium and area of cementum were calculated by computerized analysis. The data were analyzed statistically. Results 73 s was average time of extraction of treated teeth, 148 s was average of control teeth. And there was significant difference on the first premolars of both mandible and maxilla(P