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论文摘要

转化生长因子-β1 和血管内皮生长因子在兔下颌骨缺损修复中的表达

Expression of transforming growth factor-β1 and vascular endothelial growth fact

作者:方海军, 谷志远

Author:FANG Hai-jun, GU Zhi-yuan

收稿日期:2009-03-30          年卷(期)页码:2009,36(6):650-654

期刊名称:国际口腔医学杂志

Journal Name:International Journal of Stomatology

关键字:部分脱钙异种骨,骨移植,转化生长因子-β1,血管内皮生长因子,

Key words:partly demineralized xenogeneic bone, bone transplantation, transforming growth factor -β1,vascular endothelial growth factor

,

基金项目:

中文摘要

目的 观察转化生长因子(TGF)-β1 和血管内皮生长因子(VEGF)在兔下颌骨缺损修复中的表达。方法 将48 只大白兔分为Ⅰ~Ⅳ组,每组兔12 只。在Ⅰ、Ⅱ组兔的双侧下颌骨缺损区,分别植入梯度质量浓度为1 mg·L-1 和10 mg·L-1 的骨形态发生蛋白(BMP)和部分脱钙异种骨(PDXB)混合充填材料;在Ⅲ组兔的双侧下颌骨缺损区,植入同种异体骨;在Ⅳ组兔的双侧下颌骨缺损区,植入PDXB。分别于术后2、4、8 周各处死每组动物4 只,取材行免疫组化染色并观察TGF-β1 和VEGF 的表达情况,对比其相关性。结果 TGF-β1 和VEGF在Ⅲ组中表达最强,在10 mg·L-1 的PDXB-BMP 复合组的表达与Ⅲ组相近,TGF-β1 与VEGF 表达之间存在着明显的正相关关系。结论 PDXB 和BMP 混合后可显著地上调骨移植区TGF-β1 和VEGF 的表达,TGF-β1 的表达与VEGF 的表达有明显的相关性。

英文摘要

Objective The present study was designed to investigate the correlation of transforming growth factor (TGF)-β1 and vascular endothelial growth factor(VEGF) protein expression in repairing mandible defects of the rabbits. Methods The animals were divided into 4 groups randomly, each defect of two groups(the groupⅠ,Ⅱ)was grafted with bone morphogenetic protein(BMP) of different concentrations(1 mg·L-1,10 mg·L-1) reconstituted

inpartly demineralized xenogeneic bone(PDXB). In the group Ⅲ 12 rabbits(24 defects) were grafted with homogeneity bone. In the group Ⅳ 12 rabbits(24 defects) were grafted with PDXB. 16 animals were sacrificed in 2, 4

and 8 weeks after surgery respectively and the specimens were harvested and processed immuno histochemical examinations. Percentage of TGF-β1 and VEGF positive staining area were quantified by image analysis. Results

The level of TGF -β1 and VEGF protein expression was the highest in group Ⅲ , and it is similar with the expression when the concentration of BMP is 10 mg·L-1 combinated with PDXB. Furthermore, there was a close positive correlation between levels of TGF -β1 and VEGF. Conclusion The results showed that BMP reconstituted in PDXB markedly up-regulated the expression of TGF-β1 and VEGF in the grafted area. In our study, the correlation of TGF-β1 and VEGF protein expression was conspicuous, and TGF-β1 could induce VEGF protein

expression.

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