实时荧光定量聚合酶链反应检测复发性阿弗他溃疡患者中人类疱疹病毒5 型和8 型的潜伏
Employed r eal - time fluor escent quantitative polymer ized chain r eaction to q
作者：顾杨1, 李晶泉2, 尚巍1, 徐昊2, 李文颜1, 张虹1, 陈政翰2
Author：GU Yang1, LI Jing- quan2, SHANG Wei1, XU Hao2，LI Wen- yan1, ZHANG Hong1，CHEN Zheng- han2
收稿日期：2007-10-25 年卷（期）页码：2008,35(2):103-106
期刊名称：国际口腔医学杂志
Journal Name：International Journal of Stomatology
关键字：复发性阿弗他溃疡,实时荧光定量聚合酶链反应,人类疱疹病毒5 型,人类疱疹病毒8 型,
Key words：recurrent aphthous ulcer, real - time fluorescent quantitative polymerized chain reaction, human herpes virus- 5, human herpes virus- 8,
基金项目：
中文摘要
目的 采用实时荧光定量聚合酶链反应（PCR）检测轻型复发性阿弗他溃疡（RAU）患者4种样本中人类疱疹病毒5 型（HHV- 5）和8型（HHV- 8）的潜伏情况。方法 选择18 例轻型RAU 患者为试验组，同时选择健康者23 名为对照组进行临床采样，样本包括唾液、病变区脱落细胞、病变区组织块及外周血。然后用实时荧光定量PCR 检测4 种样本中HHV- 5 和HHV- 8 的DNA。结果 HHV- 5 仅在试验组患者病损区组织块样本中能检测到，而在对照组的组织块样本中没有检测到。试验组和对照组的血液和唾液样本中均有HHV- 5 阳性检出，但两组比较其差异无统计学意义（P>0.05）；试验组和对照组的脱落细胞样本中均无HHV- 5 阳性检出。试验组和对照组的4 种样本中HHV- 8 均检出阴性。结论 HHV- 5 在RAU 患者的溃疡区可能有潜伏。
英文摘要
Objective To test the DNT of human herpes virus- 5 and 8（HHV- 5 and 8）located in four kinds of samples obtained from minor recurrent aphthous ulcer（MiRAU）patients using the real- time fluorescent quantitative PCR. Methods Samples of the saliva, exfoliated cells, biopsy tissues and the blood of 18 MiRAU patients and 23 health persons were simultaneously collected in one patient or person by oral medicine specialists. They were exactly determined by the real- time fluorescent quantitative PCR. Results The HHV- 5 positive rate in MiRAU
biopsy samples had significant statistics difference with their health controls（P0.05）. In
another part, we did not find the DNA of HHV- 5 in exfoliated cells of both groups and HHV- 8 in either group. Conclusion It is supposed that HHV- 5 may be concealed in immune cells and duplicated in oral small salary gland tissue cells on account of characters of recurrent occurrence and the heal postponement in MiRAU patients.
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