Objective To analyze the effects of angiotensin（Ang）Ⅱ on the expression of osteopontin（OPN）and matrix metalloproteinase（MMP）-2 in osteoblast-like MC3T3-E1 cells and the type of receptors. Methods The mouse osteoblast -like MC3T3 -E1 cells were cultured, and divided into four groups, exposed to different concentrations of AngⅡ（10-8, 10-7, 10-6 mol•L-1） with one group as control. The secreted concentrations of OPN, MMP-2 were evaluated by enzyme-linked immunosorbent assay. In addition, the blockers of the two distinct types of receptors（AT1R and AT2R）were used to identify the receptor through which the Ang Ⅱ act in the osteoblastlike MC3T3-E1 cells. Results Ang Ⅱ, the concentration of which was within 10-8 to 10-6 mol•L-1 could increase the expressions of OPN, MMP -2 in a dose -dependent mannner. 10 -6 mol•L-1 Ang Ⅱ could stimulate the expressions of OPN, MMP-2 in a time-dependent mannner with the peak at the time of 24 h after the exposure. The AT1R blocker Losantan could significantly reduce the expressions of OPN and MMP-2 in the osteoblasts（P
