Recently, more and more researchers pay attention to plasma medicine, and the application of low-temperature plasma in dental clinical sterilization has became a new hot. Plasma sterilization, with the advantage of lowtemperature, fastness, thoroughness, safety, overcomes the deficiency of the traditional sterilization technology, and may become a novel method for killing microbe. This article was to introduce the technique of low-temperature plasma, reviewing the progress of the experimental study of the plasma disinfection and sterilization, and its dental clinical investigation. The prospect of this technique was briefly presented at the end of the article.