Objective To analyze the weak point of multiple adhesive interfaces between dentine and glass ceramic through resin cement under long-term water storage. Methods The flat dentin surfaces of 15 intact human third molars and 15 pieces of glass ceramic（ProCAD） pretreated by silane coupler were prepared. The teeth were divided into 5 groups randomly, were bonded with ceramic through etch -rinse type resin adhesive system One-step Plus +Duolinks（OD）; self-etch type resin adhesive system ED-Prime+Panavia F（EP）, Multilink Primer+Multilink Automix（MM）, and J-100P primer+J-100P Ivory（JJ）; self-adhesive type resin cement Relyx Unicem（RU）. The bonded teeth were sectioned to obtain bar -shaped intact specimens with about 0.9 mm× 0.9 mm bond area. Specimens were kept in distilled water for 18 months, and received mirco -tensile test. Results The count of fracture mode showed that ceramic interface fracture were 64% in Group OD, 57% in Group EP, 79% in Group MM， 36% in Group JJ, 39% in Group RU, while dentine interface fracture were 0% in Group OD, 14% in Group EP, 4% in Group MM, 7% in Group JJ, 14% in Group RU, and the others were the mixture fracture which all included the part of ceramic interface fracture. The bond strengths of Group OD（12.98±5.86）MPa was greater than those of Group MM（9.32±2.87）MPa（P＜0.05）, and the bond strength of Group EP, Group JJ and RU were not significantly different from Group OD and Group MM（P ＞0.05）. Conclusion The interface of resin cements and ceramics is more susceptible from water than that of resin and dentine, etch-rinse type resin bond system could obtain more stable adhesive interface.
