Objective This study was conducted to investigate the key point of nursing care during the removal of broken instrument from the root canal. Methods A total of 30 patients with broken instrument in their root canal were examined from 2011 to 2012. The patients were provided with advanced dental treatment and nursing care. Results The nurse used four-hand operating nursing care with endodontics to remove the broken instrument using GG drill and ultrasound P5 under an operating microscope, with success rates ranging from 73% to 89%. Conclusion The nursing care points during the removal of broken instrument are as follows: psychological preparation of the patient, preparation of surgical instrument (such as a microscope, GG drill, and ultrasonic P5), and intraoperative precision cooperation. Accurate, effective, and timely nursing care can improve the success rate of the broken instrument removal, shorten the operation time, and reduce the pain of patients.