Enterococcus faecalis are able to survive starvation and very harsh environment. In different part of root canal, Enterococcus faecalis vary on the planting density. Enterococcus faecalis has several virulence factors which could shift and diffuse between enterococcus, helping with nonspecific immune response -tolerance, pathogenicity and viability. This review summarizes the features of Enterococcus faecalis related to dentine tubular invasion and the detective method of it.