Objective To compare short period postoperative clinical efficacy between single-visit root canal treatment and two-visit root canal treatment and analyze different impact of different factors on curative effect. Methods Nintysix patients who needed root canal treatment, were randomly divided into two groups. One group took single-visit root canal treatment, while the other group took two-visit root canal treatment. Patients were followed up for the first 3 days and 1 week after treatment for pain and taken image examination after 3 months. According to the postoperative pain and imaging manifestations, we evaluated the postoperative clinical efficacy, then using SPSS system for data analysis and statistics. Results There was no significant difference between single-visit root canal treatment group and two-visit root canal treatment group in clinic efficacy and postoperative pain. Patients’ gender, as well as preoperative tooth periapical index(PAI), had statistical significance on the effect of root canal treatment after operation, while the patients’ age, tooth position, tooth anatomy of tooth pulp condition(no periapical lesion) showed no statistical significance. Conclusion Single-visit root canal treatment had lower postoperative pain incidence, and can be applied in clinic in some cases. Patients’ gender and preoperative PAI had influence on the efficacy of root canal treatment.