Many factors are involved in biofilm formation and disassembly. It’s a heat and new area of research about factors which can trigger biofilm disassembly and inhibit biofilm formation. Polyamines are populate in bacteria, recent research demonstrate that polyamines act on different aspects of biofilm formation and disassembly, both extracellularly and intracellularly. We provide here a brief overview of the role of polyamines in biofilm formation and disassembly.