光引发聚合改性明胶用于牙组织工程的可能性初探
Feasibility study on tooth tissue engineering using cross-linked gelatin hydrogel prepared through photo-initiated polymerization
作者：李蕾, 乔祥晨 崔彩云, 郭维华, 田卫东,
Author：Li Lei, Qiao Xiangchen, Cui Caiyun, Guo Weihua, Tian Weidong,
收稿日期：2014-10-12 年卷（期）页码：2015,42(3):265-268
期刊名称：国际口腔医学杂志
Journal Name：International Journal of Stomatology
关键字：改性明胶,光引发聚合,牙髓细胞,支架材料,组织工程,
Key words：cross-linked gelatin,photo-initiated polymerization,dental pulp cell,scaffold,tissue engineering,
基金项目：
四川省科技支撑计划（13ZC0971，13ZC0979，2013GZX 0158）
中文摘要
目的 研究光引发聚合改性明胶水凝胶的表征，理化性质以及生物相容性，为牙髓再生引入一种可能的新型复合支架材料。方法 甲基丙烯酸将明胶改性后形成甲基丙烯明胶，用核磁共振技术检测其表征，用扫描电镜观察表面形貌，甲基丙烯明胶溶液加入艳固佳2959型光引发剂，经紫外光辐照后形成水凝胶，用流变仪测量黏弹性来反映力学性能，用台盼蓝染色法和CCK-8法检测水凝胶对人牙髓细胞增殖的影响。结果 甲基丙烯酸改性明胶增加了明胶机械性能和孔隙率的同时，能够支持人牙髓细胞生长。结论 甲基丙烯明胶水凝胶有望成为人牙髓再生研究中一种有潜力的支架材料。
英文摘要
Objective The objective of this study is to determine the characteristics, physicochemical property, and bio-compatibility of gelatin methacrylate(GM) hydrogel, with an aim to introduce this as a new scaffold compound for dental pulp regeneration. Methods Gelatin(G) was cross-linked with methylacrylic acid(MA) to produce GM. Its surface appearance was assessed using scanning electron microscope(SEM), and its characteristics were tested using nuclear magnetic resonance(NMR). Hydrogel is formed by adding Irgacure2959 into the GM solution. The mixture was subsequently exposedto ultraviolet ray(UV). Rheometer was used to test viscoelastity, which reflects the mechanical property of GM. The effects of GM to the proliferation of human dental pulp cells(hDPCs) were revealed by Trypan Blue and cell counting kit-8(CCK-8). Results GM hydrogel, which was formed from cross-linking of gelatin and methylacrylic acid, not only have been improved in its mechanical property and porosity, but also supported the growth of hDPCs. Conclusion GM hydrogel is a potential scaffold material in dental pulp regeneration research.
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