Objective The study aimed to explore supragingival plaque removal efficacy of modified Bass, vertical and Fones toothbrushing method among 5 to 6 year-old children, and to compare the learn ability of the three methods. Methods Three top classes in separate kindergartens were randomly assigned to modified Bass, vertical and Fones group. Propaganda of corresponding toothbrushing method was accomplished within two days for each group. Then plaque removal efficacy was examined the second and eighth day after propaganda. Individual toothbrushing performance was graded the eighth day after propaganda. Results During the first examination, plaque removal rate of modified Bass, vertical and Fones group was 81%, 75%, 82% respectively, demonstrating no statistical significance(P>0.05). During the second examination, removal rate was 88%, 68%, 80% respectively. According to statistical analysis, modified Bass and Fones group showed better plaque removal efficacy than vertical group(P0.05). Performance score of the three groups showed no statistical significance(P>0.05). Conclusion Our results implied modified Bass and Fones method could be recommended to 5 to 6 year-old children.