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论文摘要

母子间口腔群落结构的相关性分析

Similarity analysis of the oral bacteria community by polymerase chain reaction-denaturing gradient gel electrophoresis

作者:于淼1 仪虹2 郭笑3

Author:Yu Miao1, Yi Hong2, Guo Xiao3.

收稿日期:2015-04-15          年卷(期)页码:2015,42(6):664-667

期刊名称:国际口腔医学杂志

Journal Name:International Journal of Stomatology

关键字:唾液,口腔,细菌群落,变性梯度凝胶电泳,

Key words:saliva,oral,bacteria community,denaturing gradient gel electrophoresis,

基金项目:

中文摘要

目的 应用聚合酶链反应(PCR)-变性梯度凝胶电泳(DGGE)技术,分析30对母子口腔唾液中菌群的相似性。方法 选取3~5岁高龋、中龋和无龋儿童及其母亲各10对作为研究对象,取其非刺激性全唾液,分别提取细菌总DNA,进行16S rRNA的PCR扩增及DGGE分析。结果 母子间口腔菌群组成具高度相似性。其中,无龋组母子间配对相似系数明显低于高龋组及中龋组(P

英文摘要

Objective This study aimed to analyze the similarity of the oral bacteria community in 30 mother-child dyads via polymerase chain reaction(PCR)-based denaturing gradient gel electrophoresis. Methods Unstimulated saliva samples were obtained from 30 mother-child pairs:10 in caries-susceptive group, 10 in caries-moderate group, and 10 in caries-free group. DNAs were extracted for PCR amplification and denaturing gradient gel electrophoresis analysis. Results The compositions of the oral bacteria community in mother and child have a high degree of similarity. The oral bacteria community of children matched that of their mothers. The dice similarity coefficient of mother-child in the cariesfree group was more statistically significant compared with the two other groups(P

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