牙周韧带细胞膜片技术用于牙周组织再生的研究进展

Achieving complete periodontal tissue regeneration that arises from periodontitis show some difficulties. The principles of guided tissue regeneration indicate that cell sheet engineering aims to achieve the regeneration of periodontal ligament embedded in new cementum and new alveolar bone by several novel cell-transferring Methods. For example, UpCell culture dish can control cell-surface adhesion and utilize temperature change in cell culture and a surface-grafted temperature-sensitive polymer named poly N-isopropylacylamide. Cell sheets have been harvested from temperature-sensitive culture surfaces non-invasively and overlaid as multilayered constructs. Cell sheet technique has improved in recent years and has overcome many obstacles, such as the inadequate thickness of monolayer cell sheet, the insufficient vascularization, and the lack of autologous cell sources. Owing to the high metabolic turnover rates of the periodontal ligament, many researchers have considered periodontal ligament cells as one of the most suitable candidate cell sources for regeneration. A clinical study of periodontal regeneration with autologous periodontal ligament cell sheet has been recently performed on humans in Japan. This article reviews the recent advances of techniques in periodontal ligament cell sheet engineering and its application in periodontal regeneration.