基于高通量测序技术研究慢性牙周炎患者龈下刮治和根面平整术治疗前后龈下菌群的变化

ObjectiveThis study aims to observe the changes in the microbiological community of a subgingival plaque in chronic periodondotis after subgingival scaling and root planing(SRP). The observation was performed via high-throughput sequencing. The feasibility of this approach in diagnosis and prognosis in chronic periodontitis was then explored by microflora transition.MethodsTen patients with chronic periodontitis under treatment in the Periodontics Department of Beijing Stomatological Hospital were selected in this study. From each patient, we obtained genomic DNA from the subgingival plaque before SRP and then three months after SRP in the same sampling site. High-throughput sequencing analysis was then performed to investigate the diversity and relative abundance of the subgingival plaque microflora from phyla to species.ResultsIn the phylum level, 16 phyla were detected, of which eight constitute 99% of the sequences in the subgingival plaque samples from each time point. In the genus level, 128 different genera were detected in all time points. The results showed that the relative abundance ofTannerelladecreased three months after SRP(P<0 .05), whereas those ofLeptotrichiaandStreptococcusincreased three months after SRP(P<0 .05). in the species level, 6 putative pathogens were detected. the relative abundance ofTannerella forsythiaandPrevotella intermediadecreased three months after SRP(P<0 .05).ConclusionThe microflora of a subgingival plaque in chronic periodondotis is diverse, and the relative abundance of pathogens decreased after SRP, whereas the probiotics increased. Thus, SRP can change the microbiological community.