Closing dentinal tubule is the basic guiding idedogy in the treatment of dentin hypersensitiveness(DH), but most of the traditional methods only have a short-term effect due to chew and wear of the desensitization agent coating on dentin surface. However using neodymium:yttrium-aluminum-garnet(Nd:YAG) laser in the treatment of DH can reach a more than 90% dentinal tubule closure rate, and no pits, cracks and carbonization destruction appear in dentin surface when the laser parameter is 1.5 W, 396 J·cm-2. When the temperature increment caused by laser irradiation does not exceed 5 ℃ for pulp chamber, and the increment of root surface does not exceed 10 ℃, it won’t cause any damage to the tooth pulp and periodontal tissue. Using Nd:YAG laser in the treatment of DH can reach quick and obvious curative effect, but there are also obstructive factors in the application of Nd:YAG laser such as dental fear, treatment risks, instrument limitations and the high costs. And in the process of using Nd:YAG laser, attention should be paid to the protection of eyes of patients and operators.