Objective This study aimed to investigate and compare the biological potential of two types of platelet rich plasma(PRP) loaded alginate hydrogel(PRP alginate beads and PRP alginate capsules) through animal tests in vivo. Methods A critical size defect of 15mm elongation was created in the mid-upper part of bilateral radius of 45 New Zealand white rabbits and either suppied with PRP alginate beads(14 rabbits), and PRP alginate capsules(14 rabbits), PRP gel(14 rabbits), and untreated(4 rabbits). After 8, 12 weeks of implantation, the gross, radiological and histological observations were performed to investigate the bone healing of the defect. Results All of the defects treated with transplant materials exhibited new bone formation 8 and 12 weeks postoperatively, increasing with time. The radiological and histomorphological evaluation showed that the ability of new bone formation was arranged in 3 groups as follows: PRP alginate beads>PRP alginate capsules>PRP gel, the difference was significant between them(P
