阻塞性睡眠呼吸暂停综合征对大鼠牙周组织影响的研究

摘要：目的 建立大鼠间歇低氧动物模型模拟阻塞性睡眠呼吸暂停综合征（OSAS）以及牙周炎的动物模型，探讨OSAS与牙周炎发病的相关性。方法 将20只SD大鼠随机分为常氧对照组（A组）和间歇低氧组（B组）。间歇低氧模型是将O2和N2循环通入实验舱，使O2浓度为（10%±0.5%）~（21%±0.5%），每天循环通气8 h。同时，采用正畸结扎丝结扎SD大鼠左侧上颌第二磨牙，建立牙周炎的动物模型。8周后处死动物，采用X线、组织学方法观察各组大鼠牙组织的破坏情况。结果 各组大鼠结扎侧牙相对于非结扎侧牙的牙周探诊深度、龈沟出血指数升高，牙槽骨明显吸收。B组结扎侧牙的牙周组织炎症更显著。结论 间歇低氧环境可加重牙周组织的炎症反应，这就提示OSAS可能是牙周炎的危险因素之一。

Abstract:Objective This study aims to establish the obstructive sleep apnea syndrome(OSAS) animal model in rats with periodontitis and to explore the correlation between OSAS and periodontitis. Methods Twenty SD rats were randomly divided into the control group(group A) and intermitted hypoxia exposure group(group B). The oxygen concentration in the cabin ranged from 10%±0.5% to 21%±0.5% through the circular input of oxygen and nitrogen for 8 h daily. Each SD rat received wire ligatures around the cervix of the left second maxillary molar, whereas the contralateral tooth was untreated as control. The rats were sacrificed after right weeks. X-ray and histological examination were used to evaluate the periodontal breakdown. Results The periodontal depth, sulcular bleeding index, and alveolar bone loss of the ligatured teeth significantly increased, especially in group B. Conclusion Intermittent hypoxia can aggravate periodontal inflammation and may also be a risk factor for periodontics.