端粒酶卡侯体蛋白1干扰慢病毒对口腔鳞状细胞癌细胞裸鼠致瘤作用的研究

Objective To investigate the effects of silencing telomerase Cajal body protein 1(TCAB1) on the growth of a xenograft tumor and its biological behavior. Methods Quantitative fluorescence in situ hybridization(Q-FISH) was used to detect the fluorescence intensity of the telomere in oral squamous cell carcinoma(OSCC) Cal27 cells screened stably to the 26th passage with the treatment of shTCAB1 and sh negative control(NC). The results were then compared with wild-type Cal27 cells. Then these cells with RNA interference were inoculated subcutaneously into the left and right neck of the nude mice. Tumor volume was detected periodically to draw growth curves. The weight of the tumor was calculated after executing the nude mice. The expressions of TCAB1, Bcl-2, Caspase-3, vascular endothelial growth factor(VEGF), and Cyclin D1 in the xenograft tumor tissue were examined by immunohistochemistry. Results The results of Q-FISH showed that the telomere length was significantly shorter in shTCAB1 Cal27 cells than in the shNC and wild-type Cal27 cells. The xenograft tumor in the shTCAB1 treatment group had a slower growth rate than that in the shNC group. The average weight of tumors in two groups showed that the inhibition rate was 76.9%. The immunohistochemistry results demonstrated a lower expression of TCAB1 in the shTCAB1 group. Furthermore, down-regulated expression of Bcl-2, VEGF, and Cyclin D1 and up-regulated expre-ssion of Caspase-3 were observed compared with shNC group. Conclusion Depletion of TCAB1 using the shRNA lentivirus inhibited the proliferation of the xenograft tumorin vitro. The antitumor mechanism may be associated with the apoptosis-inducing extension of cell division cycle and angiogenesis inhibition.