外排体载体给药系统的制备和体外性质的初步研究
Preparation and in vitro study of exosomes drug delivery system
作者：柯欢;龚涛;张志荣;孙逊;
Author：
收稿日期： 年卷（期）页码：2018,33(02):-143-145
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：外排体;载药系统;超速离心法;盐酸阿霉素;电穿孔法;细胞摄取;乳腺癌细胞;抗肿瘤
Key words：
基金项目：
中文摘要
目的从小鼠树突状细胞(DC2.4)中提取外排体并载入盐酸阿霉素,研究外排体的细胞摄取能力与肿瘤细胞杀伤效率。方法使用超速离心的方法从DC2.4细胞的培养基上清液中提取外排体,并通过透射电子显微镜验证提取结果;通过电穿孔法载入盐酸阿霉素并测定其粒径和Zeta电位;用超速离心法进行纯化,测定其载药量及包封率;选用小鼠乳腺癌细胞(4T1)为细胞模型,考察制剂的摄取能力和杀伤力。结果成功制备了以内源性物质外排体为载体的给药系统,相比于传统给药系统脂质体,在4T1细胞中的入胞能力以及杀伤力都有显著性增加。结论以外排体为载体的新型给药系统在未来的抗肿瘤治疗领域具有巨大的潜力。
参考文献
[1]Li JH,Sherman-Baust CA,Tsai-Turton M,et al.Claudincontaining exosomess in the peripheral circulation of women with ovarian cancer[J].BMC Cancer,2009,9:244.
[2]Sercombe L,Veerati T,Moheimani F,et al.Advances and challenges of liposomes assisted drug delivery[J].Front Pharmacol,2015,6:286.
[3]Agrawal U,Sharma R,Gupta M,et al.Is nanotechnology a boon for oral drug delivery[J].Drug Discov Today,2014,19:1530-1546.
[4]Raemdonck K,Braeckmans K,Demeester J,et al.Mergingthe best of both worlds:Hybrid lipid-enveloped matrix nanocompositesin drug delivery[J].Chem Soc Rev,2014,43:444-472.
[5]Li C,Zhang J,Zu YJ,et al.Biocompatible and biodegradable nanoparticles for enhancement of anti-canceractivities of phytochemicals[J].Chin J Nat Med,2015,13:641-652.
[6]Aryani A,Denecke B.exosomess as a nanodelivery system:A key to the future of neuromedicine[J].Mol Neurobiol,2016,53:818-834.
[7]Turturici G,Tinnirello R,Sconzo G,et al.Extracellular membranevesicles as a mechanism of cell-to-cell communication:Advantages and disadvantages[J].Am J Physiol Cell Physiol,2014,306:621-633.
【关闭】
