一种新型去滞留层HPLC柱的初步验证
Performance evaluation for a new type of removing stagnant layer HPLC column
作者：毕禄莎;李宇飞;吴熙;罗婷婷;张传维;王睿;徐小平;
Author：
收稿日期： 年卷（期）页码：2019,34(03):-172-176
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：消滞留层;新型;双电层;高效液相色谱柱;电场强度;性能;变化规律;研制
Key words：
基金项目：成都市科技局科技惠民技术研发项目(2015-HM01-00181-SF)
中文摘要
目的评价色谱柱在除去滞留层后的色谱性能变化,为新型高性能色谱柱的研究提供基础。方法采用自制非金属色谱柱,于色谱柱两端加电后,除去色谱柱固定相上的滞留层,考察加电前、后色谱柱的柱径、柱长、固定相的粒径、流速、柱温等对色谱柱保留时间、理论板数等的影响规律。结果随着色谱柱两端的电场强度升高,保留时间无显著性变化,理论板数升高,半峰宽和对称性改善;随着色谱柱的加长、柱径的增大,达到相同效果所要求的电场强度越高;另一方面,随着电场强度的进一步升高,理论板数因柱內发热而下降,出现一个最佳电场强度的极点,表现出电场强度的提升对固定相表面滞留层的破坏,可改善被分离物质在固定相中的交换,促进了色谱柱效能的提高,但过高的电场强度会造成流动相电解加剧和柱內温度上升,从而导致纵向扩散的影响升高,不利于理论板数的上升。结论首次较系统地验证了电场对滞留层的干扰与柱效能的关系,可为高效色谱柱的研制提供依据。
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