芫根总多糖对模拟高原缺氧小鼠的作用研究
Effects of total polysaccharides of Brassica rapa on hypoxic mice in simulated plateau
作者：
Author：
收稿日期： 年卷（期）页码：2020,35(02):-171-175
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：芫根总多糖;提取分离;高原缺氧;抗氧化;抗缺氧;脑;肺;急性高山病
Key words：
基金项目：
中文摘要
目的研究芫根总多糖的抗氧化作用以及对模拟高原缺氧小鼠脑、肺的保护作用。方法采用低压低氧舱模拟海拔4000 m的高原环境,评估芫根总多糖的抗高原缺氧活性。结果连续7 d低氧暴露后,与常压常氧组比较,低压低氧模型组小鼠脑组织海马区、肺组织出现明显的病理损伤,脑、肺组织中的丙二醛(MDA)、乳酸(LD)的浓度和乳酸脱氢酶(LDH)的活性显著升高,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)、ATP酶(ATPase)的活性显著降低。经过芫根总多糖给药处理后,高剂量组小鼠脑组织海马区、肺组织的病理损伤得到缓解,脑、肺组织中MDA、LDH和LD的活性显著降低,而SOD、GSH-Px、T-Aoc、ATPase的活性显著升高。结论芫根总多糖具有抗高原缺氧的作用,能保护模拟高原环境中缺氧小鼠的脑组织和肺组织,可能主要是通过升高机体自身抗氧化能力、降低氧自由基损伤、调节自身能量代谢来发挥抗高原缺氧的作用。
参考文献
[1] Patyal S,Yadav AK,Kotwal A.Changes in corneal thickness in patients with high-altitude pulmonary edema after systemic oxygen therapy[J].Indian J Ophthalmol,2018,66(11):1554-1557.
[2] Patrician A,Engan H,Lundsten D,et al.The effect of dietary nitrate on nocturnal sleep-disordered breathing and arterial oxygen desaturation at high altitude[J].High Alt Med Biol,2018,19(1):21-27.
[3] Wang W,Wang XQ,Ye H,et al.Optimization of extraction,characterization and antioxidant activity of polysaccharides from Brassica rapa L.[J].Int J Biol Macromol,2016,82:979-988.
[4] 谢玥,马超,蒋思萍,等.西藏芫根提取物对小鼠抗缺氧作用的初步研究[J].四川动物,2009,28(6):853-856.
[5] 普布多吉,陈彬,李争光,等.一组藏药抗疲劳方剂筛选及其功效研究[J].中成药,2018,39(3):696-698.
[6] Li HF,Ding F,Xiao LY,et al.Food-derived antioxidant polysaccharides and their pharmacological potential in neurodegenerative diseases[J].Nutrients,2017,9(7):E778.
[7] Nikolova M,Ambrozova G,Kratchanova M,et al.Effects of pectic polysaccharides isolated from leek on the production of reactive oxygen and nitrogen species by phagocytes[J].J Med Food,2013,16(8):711-718.
[8] Britze J,Arngrim N,Schytz HW,et al.Hypoxic mechanisms in primary headaches[J].Cephalalgia,2017,37(4):372-384.
[9] Sheppard RL,Swift JM,Hall A,et al.The influence of CO2 and exercise on hypobaric hypoxia induced pulmonary edema in rats[J].Front Physiol,2018,9:130.
[10] 任俊,马慧萍,高荣敏,等.血管内皮生长因子在急性减压缺氧小鼠肺组织中的表达研究[J].解放军医药杂志,2015,27(12):5-9.
[11] Irarrázaval S,Allard C,Campodónico J,et al.Oxidative stress in acute hypobaric hypoxia[J].High Alt Med Biol,2017,18(2):128-134.
[12] Marrocco I,Altieri F,Peluso I.Measurement and clinical significance of biomarkers of oxidative stress in humans[J].Oxid Med Cell Longev,2017:6501046.
[13] Fan PC,Ma HP,Jing LL,et al.The antioxidative effect of a novel free radical scavenger 4'-hydroxyl-2-substituted phenylnitronyl nitroxide in acute high-altitude hypoxia mice[J].Biol Pharm Bull,2013,36(6):917-24.
[14] 赵明亮,王相阳,李欣欣,等.高原缺氧代谢组学研究进展[J].中草药,2018,49(4):948-954.
[15] Hao K,Kong FP,Gao YQ,et al.Inactivation of corticotropin-releasing hormone-induced insulinotropic role by high-altitude hypoxia[J].Diabetes,2015,64(3):785-95.
[16] 景临林,武柠子,杨颖,等.黄芩素-7-甲醚对高原缺氧小鼠脑组织保护作用研究[J].天然产物研究与开发,2018,30(6):1054-1060.
[17] Paul S,Arya A,Gangwar A,et al.Size restricted silymarin suspension evokes integrated adaptive response against acute hypoxia exposure in rat lung[J].Free Radic Biol Med,2016,96:139-151.
[18] Guo P,Luo H,Fan Y,et al.Establishment and evaluation of an experimental animal model of high altitude cerebral edema[J].Neurosci Lett,2013,547:82-86.
[19] Lin H,Chang CP,Lin HJ,et al.Attenuating brain edema,hippocampal oxidative stress,and cognitive dysfunction in rats using hyperbaric oxygen preconditioning during simulated high-altitude exposure[J].J Trauma Acute Care Surg,2012,72(5):1220-1227.
[20] Nehra S,Bhardwaj V,Bansal A,et al.Nanocurcumin accords protection against acute hypobaric hypoxia induced lung injury in rats[J].J Physiol Biochem,2016,72(4):763-779.
[21] Wang C,Yan M,Jiang H,et al.Protective effects of puerarin on acute lung and cerebrum injury induced by hypobaric hypoxia via the regulation of aquaporin (AQP) via NF-κB signaling pathway[J].Int Immunopharmacol,2016,40:300-309.
【关闭】
