蜂王浆对H_2O_2所致NIH-3T3细胞损伤的修复作用及机理
Repair effects and mechanism of royal jelly on NIH-3T3 cellular damage caused by H_2O_2
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收稿日期： 年卷（期）页码：2020,35(02):-158-162
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：蜂王浆;氧化应激;NIH-3T3细胞;增殖;细胞凋亡;衰老;细胞坏死;细胞周期
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中文摘要
目的观察蜂王浆(RJ)对H_2O_2诱导的小鼠胚胎成纤维细胞(NIH-3T3)损伤的修复作用,并探讨其可能的分子机制。方法以20μg·mL(-1) H_2O_2处理NIH-3T3细胞建立细胞损伤模型,用不同浓度的蜂王浆处理模型细胞,检测各组细胞的增殖活性、β-半乳糖苷酶(SA-β-Gal)染色阳性细胞率、细胞凋亡情况以及细胞衰老相关基因的变化。结果 NIH-3T3细胞经H_2O_2处理后增殖能力下降,SA-β-Gal阳性率升高,p16、p21、p53mRNA过高表达;RJ处理后上述指标均有所改善。结论 RJ能延缓H_2O_2所致的细胞损伤和衰老。其机制可能和RJ在翻译前的环节抑制p16、p21、p53基因的表达并通过p16-cyclinD/CDK-RB和p53/P21信号通路调控细胞周期及凋亡程序有关。
参考文献
[1] Jamnik P,Goranovic D,Raspor P,et al.Antioxidative action of royal jelly in the yeast cell[J].Exp Gerontol,2007,42(7):594-600.
[2] Honda Y,Fujita Y,Maruyama H,et al.Lifespan-extending effects of royal jelly and its related substances on the nematode Caenorhabditis elegans[J].PloS One,2011,6(8):e23527.
[3] Omur G,Yusuf Kenan D,Bekir T,et al.Antibacterial effect of royal jelly for preservation of implant-related spinal infection in rat[J].Turk Neurosurg,2014,24(2):249-252.
[4] Fan P,Han B,Feng M,et al.Functional and proteomic investigations reveal major royal jelly protein 1 associated with anti-hypertension activity in mouse vascular smooth muscle cells[J].Sci Rep,2016,6:30230.
[5] Stadtman ER.Protein oxidation and aging[J].Free Radical Res Commun,2006,40(12):9-12.
[6] Kurz DJ,Decary S,Hong Y,et al.Chronic oxidative stress compromises telomere integrity and accelerates the onset of senescence in human endothelial cells[J].J Cell Sci,2004,117(11):2417-2426.
[7] Hossain M,Bhattacharjee S,Armin S,et al.Hydrogen peroxide priming modulates abiotic oxidative stress tolerance:Insights from ROS detoxification and scavenging[J].Front Plant Sci,2015,6:420-424.
[8] Mcconnell BB,Starborg M,Brookes S,et al.Inhibitors of cyclin-dependent kinases induce features of replicative senescence in early passage human diploid fibroblasts[J].Currt Biol,1998,8(6):351-354.
[9] Duan J,Zhang Z,Tong T.Senescence delay of human diploid fibroblast induced by anti-sense p16\r,INK4a\r,expression[J].J Biol Chem,2001,276(51):48325-48331.
[10] Li Y,Kokura K,Inoue T,et al.Stabilization of P/CAF,as a ubiquitin ligase toward MDM2,suppresses mitotic cell death through p53-p21 activation in HCT116 cells with SIRT2 suppression[J].Biochem Biophys Res Commun,2019,508(1):230-236.
[11] Perez SD,Silva D,Millar AB,et al.Sympathetic innervation of the spleen in male Brown norway rats:A longitudinal aging study[J].Brain Res,2009,1302:106-117.
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