HPLC荧光法测定大鼠脑组织中的盐酸多奈哌齐
Determination of Donepezil hydrochloride in rat cerebral tissues by HPLC-fluorescence method
作者：
Author：
收稿日期： 年卷（期）页码：2020,35(03):-275-278
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：乙酰胆碱酯酶抑制剂;盐酸多奈哌齐;高效液相色谱荧光法;含量测定;脑组织;药物分布;灌胃给药;大鼠
Key words：
基金项目：
中文摘要
目的测定大鼠脑组织中盐酸多奈哌齐(DPZ)的含量。方法大鼠ig给予10 mg·kg(-1) DPZ溶液,45 min后,断头处死,立即分离嗅球(OB)、嗅束(OT)、大脑(CR)及小脑(CL)等脑组织并分别称重、匀浆;采用HPLC荧光法测定大鼠脑匀浆中药物的浓度,分别计算不同脑组织中药物的含量。采用Inert Sustain C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.01 mol·L(-1) DPZ溶液,45 min后,断头处死,立即分离嗅球(OB)、嗅束(OT)、大脑(CR)及小脑(CL)等脑组织并分别称重、匀浆;采用HPLC荧光法测定大鼠脑匀浆中药物的浓度,分别计算不同脑组织中药物的含量。采用Inert Sustain C_(18)色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.01 mol·L(-1)磷酸二氢钠溶液-(30:70),激发波长325 nm,发射波长390 nm,流速1.2 mL·min(-1)磷酸二氢钠溶液-(30:70),激发波长325 nm,发射波长390 nm,流速1.2 mL·min(-1),柱温为室温,进样量20μL。结果大鼠脑匀浆中DPZ的线性范围为10.0～800.0 ng·mL(-1),柱温为室温,进样量20μL。结果大鼠脑匀浆中DPZ的线性范围为10.0～800.0 ng·mL(-1)(r=0.9994),方法的相对回收率为96.29%～104.28%,提取回收率为97.12%～104.43%(n=5),方法的日内、日间RSD均
参考文献
[1] Provensi G,Costa A,Passani MB,et al.Donepezil,an acetylcholine esterase inhibitor,and ABT-239,a histamine H3 receptor antagonist/inverse agonist,require the integrity of brain histamine system to exert biochemical and procognitive effects in the mouse[J].Neuropharmacol,2016,109:139-147.
[2] Kim YH,Choi HY,Lim HS,et al.Single dose pharmacokinetics of the novel transdermal donepezil patch in healthy volunteers[J].Drug Des Devel Ther,2015,9:1419-1426.
[3] Christodoulou C,Melville P,Scherl WF,et al.Effects of donepezil on memory and cognition in multiple sclerosis[J].J Neurol Sci,2006,245:127-136.
[4] Khan AR,Liu MR,Khan MW,et al.Progress in brain targeting drug delivery system by nasal route[J].J Contr Rel,2017,268:364-389.
[5] 谷福根,张嫒,王毅,等.盐酸文拉法辛鼻喷剂在大鼠体内的药动学研究及对鼻黏膜纤毛毒性的评价[J].华西药学杂志,2018,33(3):287-290.
[6] 樊慧敏,谷福根.鼻腔给药新剂型及临床应用进展[J].华西药学杂志,2018,33(5):548-554.
[7] Espinoza LC,Vacacela M,Clares B,et al.Development of a nasal donepezil-loaded microemulsion for the treatment of Alzheimer's disease:In vitro and ex vivo characterization[J].CNS Neurol Disord Drug Targets,2018,17(1):43-53.
[8] 包圆圆,鲍涛涛,吴慧,等.多奈哌齐经鼻给药小鼠血脑药物测定及药动学研究[J].中国医药工业杂志,2018,49(4):498-503.
[9] 李艳翠,辛华,高丽.建立HPLC-ESI-SIM法测定盐酸多奈哌齐在血浆中的含量[J].中国美容医学,2012,21(11):24-26.
[10] 戴青,陈勇川,邱学文,等.HPLC-荧光法测定氨磺必利血药浓度及其药动学研究[J].药物分析杂志,2013,33(9):1486-1490.
[11] Valis M,Masopust J,Vysata O,et al.Concentration of donepezil in the cerebrospinal fluid of AD patients:Evaluation of dosage sufficiency in standard treatment strategy[J].Neurotox Res,2017,31(1):162-168.
[12] 谷福根,马维娜,王毅,等.利培酮鼻用凝胶剂在大鼠体内的药动学及脑靶向性研究[J].中国药学杂志,2017,52(24):2179-2184.
[13] Bhavna,Shadab M,Mushir A,et al.Donepezil nanosuspension intended for nose to brain targeting:In vitro and in vivo safety evaluation[J].Int J Biol Macromol,2014,67:418-425.
[14] Groppa F,Coin A,De Rosa G,et al.Monitoring plasma levels of donepezil,5-O-desmethyl-donepezil,6-O-desmethyl-donepezil and donepezil-N-oxide by a novel HPLC method in patients with Alzheimer disease[J].Ther Drug Moint,2016,38(1):108-113.
【关闭】
