源于内生真菌的破骨细胞所形成抑制剂的研究
Study on the osteoclastogenesis inhibitors from an endophytic fungus
作者：褚以文;李端华;余蓉;
Author：
收稿日期： 年卷（期）页码：2011,26(03):-201-203
期刊名称：华西药学杂志
Journal Name：WEST CHINA JOURNAL OF PHARMACEUTICAL SCIENCES
关键字：洛伐他汀类似物;破骨细胞;骨吸收抑制剂;土曲霉;内生真菌
Key words：
基金项目：科技基础条件平台项目(No.2005DKA21203);;
四川省科技条件平台专项
中文摘要
目的分离鉴定由梨果仙人掌内生土曲霉SIIAF5446菌株产生的破骨细胞所生成的抑制剂。方法采用溶剂萃取、硅胶柱层析、反相制备HPLC来分离纯化发酵液,借助光谱学手段鉴定其结构,并采用抗酒石酸磷酸酶阳性多核细胞计数法测定其抑制小鼠骨髓破骨细胞生成的活性。结果分离得到3个抑制破骨细胞生成的高活性化合物,即洛伐他汀、3″-羟基洛伐他汀和2″,3″-脱氢洛伐他汀。结论 3″-羟基洛伐他汀和2″,3″-脱氢洛伐他汀为活性新化合物。
英文摘要
[1]Takahashi N,Yaman H,Yoshiki S,et al.Osteoclast-like cellformation and its regulation by osteotrophic hormones in mousebone marrow cultures[J].Endocrinology,1988,122:1373-1382.
[2]Klich MA,Pitt JI.A laboratory guide to common Aspergillus spe-cies and their teleomorphs[M].CSIRO Division of Food Re-search,North Ryde,NSW,Australia,1988.
[3]Wang H,Wu YH,Zhao ZX.Fragmentation study of simvastatinand lovastatin using electrospray ionization tandem mass spec-trometry[J].J Mass Spectrom,2001,36:58-70.
[4]Endo A,Hasumi K,Nakamura T,et al.Dihydromonacolin L andmonacolin X,new metabolites those inhibit cholesterol biosynthe-sis[J].J Antibiotics,1985,38:321-327.
[5]Mundy G.Stimulation for bone formation in vitro and in rodentsby statins[J].Sci,1999,286(5446):1946-1949.
[6]Hughes A,Rogers MJ,Idris AI,et al.A comparison between theeffects of hydrophobic and hydrophilic statins on osteoclast func-tion in vitro and ovariectomy-induced bone loss in vivo[J].Cal-cif Tissue Int,2007,81(5):403-413.
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