Objective To investigate the effective method of treating dentine hypersensitivity. Methods 356 teeth of dentine hypersensitivity in 120 patients were randomly divided into experimental group and controll group. Teeth in experimental group were treated for 3 days by Prime&Bond R NT bonding agent and teeth in controll group were routinely treated for 3 days by herbal desensitizer respectively. The clinical effects were observed in the following 6 months. Results All the effects including immediate effect, effect of 3 months and effect of 6 months of experimental group were better than that of controll group. Conclusion Prime&Bond RNT bonding agent is an ideal desensitizer in the treatment of dentine hypersensitivity.