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论文摘要

周期性张应力对牙周膜成纤维细胞增殖的影响

Effect of cyclic stretch on human periodontal ligament fibroblast proliferation

作者:邵扬1 张广耘2 袁晓2

Author:Shao Yang1, Zhang Guangyun2, Yuan Xiao2.

收稿日期:2010-12-25          年卷(期)页码:2011,38(4):384-387

期刊名称:国际口腔医学杂志

Journal Name:International Journal of Stomatology

关键字:周期性张应力, 人牙周膜成纤维细胞, 增殖, 细胞计数试剂-8, 核因子-&kappa,B,

Key words:cyclic stretch; human periodontal ligament fibroblast; proliferation; cell counting kit-8; nuclear factor-&kappa,B,

基金项目:

国家自然科学基金资助项目(30770528)

中文摘要

目的 探讨周期性张应力对人牙周膜成纤维细胞(hPDLF)增殖的影响及其机制。方法 采用多通道细 胞牵张应力加载系统,以hPDLF 为对象构建细胞体外培养-力学刺激模型。加力组分别给予1、2、4、6、12和 24 h 的力学刺激,刺激幅度10%、频率为0.1 Hz。以静态组为对照组。应用细胞计数试剂-8(CCK-8)检测 hPDLF 增殖情况,RT-PCR 检测增殖性细胞核抗原(PCNA)和核因子-κB(NF-κB)的表达;以NF-κB 特异性抑制 剂吡咯烷二硫代氨甲酸盐(PDTC)预处理细胞作为对照组,检测hPDLF 增殖情况。结果 加力组细胞在加力1、 2 h 增殖下降,6 h 开始增殖,12 h 增殖达高峰,24 h 增殖受到明显的抑制;PDTC 抑制细胞增殖以及pcna 和nf- κB mRNA 的表达。结论 在一定的时间范围内,周期性张应力能促hPDLF 增殖;随着时间的延长,细胞增殖 受抑制;NF-κB 信号传导通路在周期性张应力介导的hPDLF 增殖中发挥着重要的作用。

英文摘要

Objective To study the cyclic stretch on human periodontal ligament fibroblasts(hPDLF) prliferation and its mechanism. Methods In vitro culture -tensile stimulate models of hPDLF were established by using a multi-passage load adding system. Cyclic stretch was applied on the fibroblasts for 1, 2, 4, 6, 12 and 24 hours, respectively, the load was set for 10% surface elongation, with frequency 0.1 Hz. At the same time, the normal control group was established by static group. The cell promote was determined by cell counting kit-8(CCK-8) method; and the expression of mRNA was detected on proliferating cell nuclear antigen(PCNA) and nuclear factor- κB(NF-κB) by RT-PCR technology. Results Compared with static group, the proliferation activity of hPDLF was decreased in 1, 2 h; hPDLF began to proliferate in 6 h, cell proliferation reached the peak in 12 h; the proliferation of hPDLF were inhibited obviously. Pyrrolidine dithiocarbamate(PDTC) inhibited cell proliferation and inhibited mRNA expression of PCNA and NF-κB;and the differences between the two groups were significant. Conclusion In a certain time, within the scope of cyclic stretch can promote the in vitro hPDLF proliferation; as time went by, the hPDLF was obvious inhibition. NF-κB signaling pathway play an important role during cyclic stretch-induced hPDLF proliferation.

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