不同种植方式的种植体-骨界面基质金属蛋白酶2的表达变化
Matrix metalloproteinase 2 expression in implant-bone interface remodeling influenced by submerged and nonsubmerged implant denture
作者：袁林，陈勰，程峰，杨征毅，潘广嗣，王涵，孙晋，曹依娜
Author：Yuan Lin, Chen Xie, Cheng Feng, Yang Zhengyi, Pan Guangsi, Wang Han, Sun Jin, Cao Yina
收稿日期： 年卷（期）页码：2014,41(6):631-634
期刊名称：国际口腔医学杂志
Journal Name：International Journal of Stomatology
关键字：埋植型,非埋植型,种植体-骨界面,受载,改建,基质金属蛋白酶2,
Key words：submerged,nonsubmerged,implant-bone interface,loading,remodeling,matrix metalloproteinase 2,
基金项目：
中文摘要
目的 对比埋植型与非埋植型种植体在义齿修复受载后，种植体-骨界面中基质金属蛋白酶2表达变化的异同。方法 选用Beagle犬8只，在其下颌分别植入埋植型与非埋植型种植体，采用固定金属全冠行种植义齿修复，分不同时期处死动物。采用免疫组织化学方法，对修复后不同时期埋植型与非埋植型种植体-骨界面基质金属蛋白酶2进行动态观察，比较两者之间的异同。结果 种植义齿修复受载后，随着种植体周围骨组织发生改建，种植体-骨界面表达基质金属蛋白酶2较未修复对照组明显增强。2周时种植体-骨界面基质金属蛋白酶2有表达，但与对照组无明显差异。4周和8周时明显高于对照组，并于4周时达到高峰。12周时恢复到接近对照组水平。所有埋植型和非埋植型种植体之间无显著差异。结论 埋植型和非埋植型种植体在义齿修复受载后，所承受的机械负荷均能经过种植体传递至周围骨组织刺激种植体-骨界面基质金属蛋白酶2表达的变化，促进其界面的改建。埋植型和非埋植型种植义齿受载后其界面的改建之间无明显差异。
英文摘要
Objective This study was designed to investigate the matrix metalloproteinase 2(MMP2) expression in implant-bone interface remodeling after loading the submerged and nonsubmerged implant dentures. Methods Eight adult beagle dogs were used to construct the animal model. Submerged and nonsubmerged implants were implanted into the bilateral mandible. Fixed metal full crown was used to load the submerged and nonsubmerged implant denture. The beagle dogs were sacrificed after loading. Immunohistochemistry was used to observe the dynamic MMP2 expression in the implant-bone interface. Results MMP2 expression in the implant-bone interface of the experimental group was significantly enhanced compared with that of the control group after loading of implant denture. MMP2 expression in the interface was observed 2 weeks after implant denture loading, but no statistically significant difference was found between the experimental and control groups. MMP2 expression in the experimental group was significantly higher than that in the control group at 4 and 8 weeks after loading, and the peak was reached at 4 weeks. Then, the MMP2 expression in the experimental group declined to the level of the control group after 12 weeks. No obvious difference was observed in the MMP2 expression in the implant-bone interface between the submerged and nonsubmerged implant denture. Conclusion The mechanical stress that was transferred from the implant to the bone tissue after loading of the submerged and nonsubmerged implant denture can stimulate MMP2 expression in the implant-bone interface, promoting interface remodeling. Furthermore, no distinct difference was observed in the implant-bone interface remodeling after the loading of submerged and nonsubmerged implant denture.
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