Objective To explore the effect of nifuroxazide on proliferation, migration, and invasion of thyroid papillary carcinoma cells. Methods BCPAP and TPC-1 cell lines treated with different concentration (0, 1.25, 2.5, 5, 10, 20 μmol/L) of nifuroxazide, respectively. Cell viability and proliferation of BCPAP and TPC-1 was evaluated by MTT and colony formation assay. Apoptosis analysis and cell nuclear changes were determined by staining with Hoechst 33258 and visualized by a fluorescence microscope after treatment with nifuroxazide. Western blot analysis was used to evaluate protein expressions of apoptosis and invasion of BCPAP cells treated (48 h) with nifuroxazide. Transwell assay was conducted to evaluate ability of cell migration and invasion. Results After being treated with nifuroxazide (0, 1.25, 2.5 μmol/L and 0, 1.25 μmol/L) for 24, 48, 72 h respectively, decreased proliferations of BCPAP and TPC-1 cell lines were not obvious ( P>0.05). However, treated BCPAP and TPC-1 cells with higher concentration respectively (5, 10, 20 μmol/L and 5,10 μmol/L) of nifuroxazide for 24, 48, 72 h, the inhibitory effects were significantly obvious ( P P P P P P P P Pin vitro, and blocks migration and invasion of cells in vitro by reducing protein expressions of MMP-2 and MMP-9.
