ObjectiveTo establish animal model of tunica media-induced systemic inflammatory responses and verify the stability of established model.MethodsTunica media of aorta was captured from C57/BL6 mouse donor, followed by the intervention of homogenization. After centrifugation, the supernatant was harvested and injected into the acceptor of C57/BL6 mouse to mimic the featured pathological changes induced by the exposure of tunica media with circulation. ELISA assay was used to detect the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6).ResultsThe levels of TNF-α, IL-6 in the model group were significant higher than those in the control group (PConclusionThe exposure of tunica media to circulation may induce systemic inflammatory responses.
