miRNA-221在EMS组织及间质细胞中的表达及对细胞增殖的影响
The Expression of microRNA-221 in Endometriosis and Its Impact on Endometrial Stromal Cells
作者：杜小航, 石钢, 吕东昊, 王宇翮, 陈洁婷, 郑倩, 尹霞
Author：DU Xiao-hang, SHI Gang, LÜ Dong-hao, WANG Yu-he, CHEN Jie-ting, ZHENG Qian, YIN Xia
收稿日期：2018-01-02 年卷（期）页码：2018,49(4):546-550
期刊名称：四川大学学报（医学版）
Journal Name：JOURNAL OF SICHUAN UNIVERSITY （MEDICAL SCIENCE EDITION）
关键字：子宫内膜异位症, microRNA-221, <i>PTEN</i>, 细胞增殖
Key words：Endometriosis, microRNA-221, PTEN, Cell proliferation
基金项目：
中文摘要
目的 探讨microRNA-221（miRNA）在子宫内膜异位症（endometriosis，EMS）患者组织及细胞中的表达及对EMS间质细胞增殖的影响。 方法 收集非EMS患者在位内膜（正常子宫内膜）及EMS患者卵巢异位内膜，分离培养及鉴定子宫内膜间质细胞，采用茎环法实时荧光定量PCR（qRT-PCR）检测在位、异位内膜组织及间质细胞中miRNA-221的表达；雌激素（17β-E2，终浓度为10-8mol/L）刺激间质细胞48 h后检测miR-221-3p表达变化；异位间质细胞转染miR-221-3p inhibitor和inhibitor阴性对照（NC）后，观察其对miR-221-3p、人第10号染色体缺失的磷酸酶及张力蛋白同源基因（PTEN）表达和增殖的影响。 结果 miR-221-3p在EMS组织中的表达为正常子宫内膜组织的4.2倍（P=0.039），在EMS间质细胞中的表达为正常子宫内膜间质细胞的2.66倍（P=0.029）。与正常子宫内膜组织及间质细胞相比，miR-221-5p在EMS组织及间质细胞中表达差异均无统计学意义（P>0.05）。雌激素处理正常及异位间质细胞48 h后miR-221-3p表达差异无统计学意义。抑制miR-221-3p功能后，异位间质细胞增殖抑制（P=0.018），PTEN基因表达上调（P=0.021）。 结论 miRNA-221在EMS组织及间质细胞中表达上调，抑制miR-221-3p功能可促进PTEN表达从而抑制EMS间质细胞增殖。
英文摘要
ObjectiveTo determine the expression of microRNA-221 (miR-221) in endometrial tissues and its impact on the proliferation of ectopic endometrial stromal cells.MethodsEndometrial stromal cells were isolated, cultured and identified from normal endometrial tissues (taken from patients without endometriosis) and ectopic endometrial tissues (taken from patients with ovarian endometriosis). The expression of microRNA-221 was detected by stem-loop qRT-PCR. Changes in the expression of miR-221-3p in endometrial stromal cells exposed to estraldiol (10-8mol/L) for 48 h were detected. The effects of miR-221-3p inhibitor on the expressions of miR-221-3p, phosphatase and tensin homology deleted on chromosome ten (PTEN) and cell proliferations were compared with those of the negative control (NC, 10 nmol/L).ResultsThe expression of miR-221-3p in ectopic endometrial tissues was 4.2 times higher than that in normal endometrial tissues (P=0.039):2.66 times higher in ectopic endometrial stromal cells compared with normal endometrial stromal cells (P=0.029). But no differences in the expression of miR-221-5p were found (P>0.05). No differences in the change of miR-221-3p expression after exposure to estrogen for 48h were found between normal and ectopic stromal cells. Inhibition of miR-221-3p function was associated with decreased cell proliferation (P=0.018) and increased expression ofPTENgene (P=0.021).ConclusionThe expression of microRNA-221 is upregulated in ectopic endometrial tissues and ectopic endometrial stroma cells. Inhibiting the function of miR-221-3p may result in increasedPTENexpression and decreased cell proliferation in endometrial stromal cells.
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