Objective To compare the two sequences 〔single shot true-FISP-PSIR (single shot-PSIR) and segmented-turbo-FLASH-PSIR (segmented-PSIR)〕 in the value of quantification for myocardial infarct size at 3.0 tesla MRI. Methods 38 patients with clinical confirmed myocardial infarction were served a comprehensive gadonilium cardiac MRI at 3.0 tesla MRI system (Trio, Siemens). Myocardial delayed enhancement (MDE) were performed by single shot-PSIR and segmented-PSIR sequences separatedly in 12-20 min followed gadopentetate dimeglumine injection (0.15 mmol/kg) .The quality of MDE images were analysed by experienced physicians. Signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR) between the two techniques were compared. Myocardial infarct size was quantified by a dedicated software automatically (Q-mass, Medis). Results All objectives were scanned on the 3.0T MR successfully. No significant difference was found in SNR and CNR of the image quality between the two sequences (P>0.05), as well as the total myocardial volume, between two sequences (P>0.05). Furthermore, there were still no difference in the infarct size 〔single shot-PSIR (30.87±15.72) mL, segmented-PSIR (29.26±14.07) mL〕, ratio 〔single shot-PSIR (22.94%±10.94%), segmented-PSIR (20.75%±8.78%) 〕 between the two sequences (P>0.05). However, the average aquisition time of single shot-PSIR (21.4 s) was less than that of the latter (380 s). Conclusion Single shot-PSIR is equal to segmented-PSIR in detecting the myocardial infarct size with less acquisition time, which is valuable in the clinic application and further research.