脑缺血肺损伤大鼠肺组织TNF-α的表达变化

Expression of TNF-α in Lung Tissue of Rats with Lung Injury Induced by Brain Ischemia

作者：廖凤, 但齐琴, 杜润芬等

Author：LIAO Feng, DAN Qi-qin, DU Run-fen. et al

收稿日期： 年卷（期）页码：2012,43(6):914-918

期刊名称：四川大学学报（医学版）

Journal Name：JOURNAL OF SICHUAN UNIVERSITY （MEDICAL SCIENCE EDITION）

关键字：脑缺血肺损伤肿瘤坏死因子-α 大鼠

Key words：Brian ischemia lung injury TNF-α Rat

基金项目：

中文摘要

【摘要】目的探讨脑缺血后损伤肺组织肿瘤坏死因子-α（TNF-α）的表达变化。方法成年SD大鼠随机分为假手术组、脑缺血肺损伤组(n=13)，其中每组5只用于免疫组化检测TNF-α在肺的定位分布，余8只分别用于RT-PCR和Western blot检测TNF-α mRNA和蛋白水平变化。术后24 h取肺组织行RT-PCR、术后48 h取肺组织行Western blot检测肺组织TNF-α mRNA和蛋白表达水平；术后3 d取肺组织行免疫组织化学染色检测肺组织内TNF-α的定位分布。结果肺组织TNF-α mRNA和蛋白表达在脑缺血肺组织明显增加，与假手术组比较，差异有统计学意义（P

英文摘要

【Abstract】 Objective To investigate the expression of tumor necrosis factor-α (TNF-α) in the lung tissue of rats with lung injury induced by brain ischemia. Methods The rat model of lung injury induced by brain ischemia was established. At 24 h, 48 h, 72 h after brain ischemia, lung tissues were harvested from each rats, the expressions of TNF-α mRNA and protein and its distributions in the lung tissue were measured by the methods of RT-PCR （n=8）, Western blot （n=8）, and immunohistochemistry （n=5）, respectively. Results The increase of TNF-α mRNA and protein levels were found in the lung tissues after brain ischemia, when compared with sham group （P

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论文摘要

脑缺血肺损伤大鼠肺组织TNF-α的表达变化

Expression of TNF-α in Lung Tissue of Rats with Lung Injury Induced by Brain Ischemia