ObjectiveTo investigate the regulation effect of Morusin on stemness phenotype of laryngeal cancer stem cells.MethodsSeparation and detection the proportion of CD133+laryngeal cancer stem cells through flow cytometry; evaluation the self-renewal ability of CD133+laryngeal cancer stem cells by tumor sphere formation assay; exploring the migration ability of CD133+laryngeal cancer stem cells by Transwell assay; analyzing the cytotoxicity of chemotherapy drugs on CD133+laryngeal cancer stem cells by modified MTT assay; detection of the expression levels of stemness associated markers by immunofluorescence staining, RT-qPCR and Western blot. After treatment with different concentrations of Morusin, cells were performed the above experiments for detection the self-renewal ability, migration ability, cytotoxicity resistance and expression of stemness associated markers.ResultsFlow cytometry analysis showed that the proportion of CD133+laryngeal cancer stem cells was (3.50±0.34)%, while after enrichment, the proportion increased to (93.20±5.23)%. CD133+laryngeal cancer stem cells exhibited better self-renewal ability (P<0 .001) and migratory ability (P<0 .05); they were resistant to the cytotoxicity of chemotherapy drug (P<0 .05), and highly expressed of stemness associated markers. after being treated with morusin, the self-renewal and migratory abilities of cd133+laryngeal cancer stem cells were reduced (P<0 .05). in addition, after treated with morusin, cd133+laryngeal cancer stem cells were more sensitive to chemotherapy drugs; moreover, the expression levels of stemness associated markers were decreased.ConclusionCD133+laryngeal cancer stem cells possessed stemness phenotypic characteristics. Morusin attenuated stemness phenotype of laryngeal cancer stem cells, which may be related to its down-regulation effect on stemness associated markers.
