In the DNA triangle nanostructure, its corner is composed of Holliday junction in DNA homologous recombination.To further investigate it，the DNA triangle was modified by introducing the sticky end to the holiday junction motif to make it self assemble into a DNA nanostructure.The gel electrophoresis analysis showed that the migration rate of this new DNA nanostructure was faster than that of the DNA triangle. Without the distortion，the motif assembled to form a dimer structure instead of a trimer triangle. The DNA nanostructure was crystallized and modified with selenium to obtain high quality crystals. The DNA nanostructure and the structure of the Holliday motif under non-twisting tension will be further studied and determined in order to offer more insights into DNA nanomaterial interaction and self-assembly.
