In order to expand the cultivation substrate of Ganoderma lucidum and apply the new strain, the method of cultivating Ganoderma lucidum with juncao and analyzing its protein, amino acid, polysaccharide and triterpene was used. The cultivation characteristics and nutrient and effective active components of introduced Ganoderma lucidum strain Nan-GL11 were analyzed. The results indicated that when Nan-GL11 and HanZhi-1 were cultivated with antlers Ganoderma lucidum, Nan-GL11 was antler like, while the main cultivated variety HanZhi-1 was bead-like, and the shape of Nan-GL11 was superior to HanZhi-1. The stipe of Nan-GL11 was significantly longer than that of Hanzhi-1 by 7.4%. The stipe diameter of Nan-GL11 was slightly thicker than that of Hanzhi-1, but without significant difference. The fresh and dry yield of Nan-GL11 was significantly higher than Hanzhi-1 by 40.9% and 21.4%, respectively. Their fresh-dry ratio was 2.55 and 2.18, respectively. The contents of polysaccharide and total triterpenoid in antler-shape Nan-GL11 cultivated with Juncao were higher than that in Nan-GL11 by 28.57% and 10.2%, respectively, and with a significant difference between them. The contents of crude protein and amino acid in Nan-GL11 was slightly lower than that in Hanzhi-1 but without significant difference, and they had the same amino acid components with 17 kinds of amino acid. Tryptophan was unable to be checked out probably because of the acid decomposition. Because of good shape, high yield and effective active ingredients of the Nan-GL11 antlers when cultivated, antler-shape Nan-GL11 has good prospects for popularization.