高表达miR-122腺病毒载体构建与功能检测
Construction and identification of miR-122 expressing recombinant adenovirus
作者：郝军莉(成都医学院生物医学系)；张金华(成都医学院生物医学系)；吴祥云(成都医学院生物医学系)；何茂兰(成都医学院生物医学系)；王丹(成都医学院生物医学系)
Author：HAO Jun-Li(School of Biomedical Sciences, Chengdu Medical College)；ZHANG Jin-Hua(School of Biomedical Sciences, Chengdu Medical College)；WU Xiang-Yun(School of Biomedical Sciences, Chengdu Medical College)；HE Mao-Lan(School of Biomedical Sciences, Chengdu Medical College)；WANG Dan(School of Biomedical Sciences, Chengdu Medical College)
收稿日期：2014-11-26 年卷（期）页码：2016,53(2):424-429
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：miR-122，腺病毒，高表达，高纯度，抗乙肝病毒活性
Key words：miR-122, Adenovirus virus, overexpression, high purity, anti-HBV activities
基金项目：其它
中文摘要
旨在利用改造后的腺病毒表达载体pDC312-cmv，构建含有8个miR-122表达框的miR-122高表达重组载体p-8miR-122，通过RT-PCR、ELISA、Sourthern blot验证重组载体高表达miR-122的水平以及抗乙肝病毒活性的能力. 通过腺病毒的包装、扩增、纯化和滴度检测，获得高纯度的腺病毒并成功检测到miR-122高表达和抗乙肝病毒活性的能力.
英文摘要
To construct miR-122 overexpression Adenovirus vector，gene including eight miR-122 precursor fragments was inserted into recombinant construct pDC312-cmv. Abilities of the recombinant vector p-8miR-122 to express miR-122 level and anti-HBV activities were analysed by RT-PCR、ELISA、Sourthern blot. Through adenovirus packaging、amplification、purification and titer detection，the pDC312-cmv and p-8miR-122 adenovirus had a high purity，high miR-122 expression levels and significantly anti-HBV activities.
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