In plants, multiple floral induced-pathways include vernalization signaling, gibberellin signaling, autonomous pathway and photoperiod signaling.The fbn1a/fbn8 double-mutant of Arabidopsis thaliana is acquired to study the effects of flowering time.In this study, the spatiotemporal expression pattern for FBN1a and FBN8, responses of its mutant to environmental factors, and its regulation of mechanism of flowering time were investigated.The mutants flowered earlier than Col-0 under long day (LD) or short day (SD) conditions. Moreover , the early flowering time of fbn1a /fbn8 double-mutant was more obvious. Gene FBN1a and FBN8 may play a cooperative role in control of flowering time. Compared with the single mutant,the expression levels of SOC1、FT and CO in double-mutant is regulated by the continuous detection of key genes, whereas the expression level of FLC decreased. H2O2 in fbn8,fbn1a and fbn1a /fbn8 increased 20%、30%、50%. The experimental results suggested that Fibrillins may affect the content of H2O2 the flowering time. Key words: flowering regulation pathways, double-mutant, Arabidopsis thaliana, H2O2
