The MYB transcript factors widely involves in many important physiological processes in plants, including cell differentiation, cell cycle regulation, hormone and environmental stress responses, secondary metabolism, and tissue morphogenesis. A fragment of MYB gene was figured out based on transcription analysis of different periods of stem swelling. Two cDNA sequences, 975bp and 864bp, were cloned by rapid amplification of cDNA ends. Moreover, these two genes, namely BjMYB1-3 and BjMYB1-4, may be the two different alternative splicing patterns of BjMYB1 gene. By analysis the tissue expression of BjMYB1-3 and BjMYB1-4, they were particularly expressed in stem. An analysis of the subcellular localisation of the proteins indicated that BjMYB1-4 was present only in the nucleus, while the BjMYB1-3 GFP fusion protein was partially localised to the nucleus. By constructing the overexpression vector of BjMYB1-3 and BjMYB1-4 and conducting the genetic transformation of Arabidopsis thaliana, we found that the plants which were transformated taked out and flowered in advance. The result showed that these two transcripts played an important role in the growth and development of plant. The result of study laid the foundation of clarifying the function of MYB gene.
