“三月花”黄花菜的组织培养研究
Study on tissue culture of Hemerocallis citrine Baroni ‘March Flower
作者：王静(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；胡冬梅(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；侯静(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)；白洁(四川大学生命科学学院 生物资源与生态环境教育部重点实验室)
Author：WANG Jing(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；HU Dong-Mei(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；HOU Jing(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)；BAI Jie(Key Laboratory Bio-resources and Eco-environment of Ministry Education,College of Life Sciences,Sichuan University)
收稿日期：2018-02-03 年卷（期）页码：2019,56(1):167-172
期刊名称：四川大学学报: 自然科学版
Journal Name：Journal of Sichuan University (Natural Science Edition)
关键字：“三月花”黄花菜; 幼叶; 愈伤组织途径; 快繁
Key words：Hemerocallis citrine Baroni‘March Flower’; Young leaves; Callus pathway; Tissue culture
基金项目：黄花菜早熟新品种“三月花”种苗标准化繁育和规模化种植示范科技合作专项资金(20826041A4117)；“三月花”黄花菜快繁技术横向(16H0487)
中文摘要
采用单因素和正交实验方法对“三月花”黄花菜的组织培养技术进行了探索.结果显示:幼叶用0.1 %升汞灭菌12 min时效果最佳,外植体污染率、死亡率均为0, 14 d后外植体启动率为62.5 %.最佳愈伤诱导培养基为MS + 2 mg/L 2,4-D + 0.1 mg/L 6 BA,外植体出愈率为86.67 %;愈伤组织最适分化培养基为MS + 2 mg/L 6-BA + 0.5 mg/L IBA,愈伤组织分化率达到80 %,平均每块愈伤组织不定芽个数为1.72.最佳生根培养基为1/2 MS+0.5 mg/L NAA, 生根率达到 93.33 %,平均每个芽苗生根数为5.83条,试管苗炼苗移栽后,成活率达 95 %.利用该组织培养技术,幼叶外植体通过愈伤组织途径获得“三月花”黄花菜再生苗.
英文摘要
Single factor and orthogonal experiment methods were used to explore the tissue culture technique of Hemerocallis citrine ‘March Flower’. The results showed that the best effect was when the young leaves were treated with 0.1 % mercuric chloride for 12 min with no pollution and death and the activation rate was 62.5 % after 14 days. MS + 2 mg/L 2,4-D + 0.1 mg/L 6-BA was the optimal induction medium with the 86.67 % recovery rate, MS + 2 mg/L 6-BA + 0.5 mg/L IBA was the best differentiation medium with the 80 % differentiation rate, and the average number of adventitious buds per block was 1.72. 1/2 MS + 0.5 mg/L NAA was the best rooting medium, the rooting rate was 93.33 %, the average number of rooting per shoot was 5.83. The survival rate of transplanted test tube seedling was 95 %. Using the tissue culture technique, the young leaf explants obtained the "March flower" daylily regeneration seed through the callus pathway.
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